Introduction
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GelQuest - DNA Fingerprint Analysis Software
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GelQuest is a stand-alone application for the analysis of DNA
fingerprint profiles and fragment patterns. The data may come from
automatic sequencers or from conventional gel images. GelQuest
carries out the following steps: trace data processing (smoothing,
baselining), peak detection, sizing, binning and binary matrix (01-matrix) construction.
The program runs on Microsoft Windows operating systems.
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ClusterVis is an application that analyses
binary matrix data (01
matrix), e.g. as generated by GelQuest. It can be started directly from
GelQuest or it can be used as a standalone program to analyse
binary data generated by other software.
ClusterVis calculates distance matrices using many different distance
measures. It calculates cluster trees (neighbor-joining, UPGMA) with
or without statistical support (jackknife, bootstrap). It also performs
principal coordinates analysis for a quick identification of outliers.
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GelQuest and ClusterVis are recommended for the analysis of
· AFLP (amplification fragment length polymorphism)
· t-RFLP (terminal restriction fragment length polymorphism)
· RFLP (restriction fragment length polymorphism)
· RAPD (random amplified polymorphic DNA)
· microsatellite analysis
· PFGE (pulse field gel electrophoresis)
· etc.
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