Trace processing and peak detection
|
Previous Top Next |
Step
|
Description
|
Trace data processing
and peak detection.
|
The parameters for these analyses are defined in the upper
part of the GelQuest analysis window.
'Trace data processing' means that the original curve values
('raw trace data') are smoothed and that the background
noise is reduced by a base lining procedure. This step
generates 'processed data' data from the 'raw data'.
This requires two parameters:
Smoothing half width:
If the smoothing half width is set to zero, no baselining will be
carried out.
Baselining half width:
If the baselining half width is set to zero, no baselining will be
carried out.
The 'peak detection' procedure analyses the 'processed data'
and determines which local maxima are considered to be
peaks.
The Gelquest Peak Digger alogrithm is a recursive local
maxium finder using residuals.
If you are a beginner to fragment analysis, it is recommended
to use the parameter 'Min. Peak Height' only. If you are an
experienced user you may wish to use the other and more
sophisticated parameters that are available.
The following parameters are available for peak detection:
The results of these steps can be seen ("preview") before
performing the last step:
|
Sizing of fragments
|
This step determines the size of each fragment by using a set
of predefined fragments from a size standard lane ('size
standard template').
The parameters for sizing are defined in the lower part of the
GelQuest analysis window.
If you are using a size standard that is not already saved as a
size standard template, you need to define a new size
standard template.
|